Key Takeaways: Cell Structure
1. Prokaryotic vs. Eukaryotic Cells
- Prokaryotic Cells (e.g., bacteria):
- No nucleus (DNA in a single loop/plasmids).
- Smaller (1–10 µm).
- Cell wall (not cellulose), cytoplasm, ribosomes.
- Eukaryotic Cells (plants, animals, fungi):
- Have a nucleus and membrane-bound organelles.
- Larger (10–100 µm).
- Plant cells: Cellulose cell wall, chloroplasts, vacuole.
2. Animal vs. Plant Cells
Animal Cells | Plant Cells |
---|---|
No cell wall | Cellulose cell wall |
No chloroplasts | Chloroplasts (for photosynthesis) |
Small/transient vacuoles | Large permanent vacuole |
Irregular shape | Regular shape (due to wall) |
Shared Features: Nucleus, mitochondria, ribosomes, cytoplasm, cell membrane.
3. Specialized Cells & Adaptations
- Sperm Cell: Tail (movement), mitochondria (energy), streamlined.
- Nerve Cell: Long axon, myelin sheath (fast impulses), dendrites (signal reception).
- Red Blood Cell: Biconcave shape (maximizes oxygen), no nucleus.
- Root Hair Cell: Long extension (increases surface area for absorption).
- Xylem: Hollow, lignin-strengthened tubes (water transport).
- Phloem: Sieve plates (translocate sugars), companion cells.
Cell Differentiation:
- Cells specialize during development (e.g., muscle cells develop more mitochondria).
- Plants retain differentiation ability throughout life.
4. Microscopy
- Light Microscope:
- Magnification: ~×1,500.
- Resolution: ~200 nm.
- Uses light; can view live cells.
- Electron Microscope:
- Magnification: Up to ×2,000,000.
- Resolution: 0.1 nm.
- Types: TEM (internal detail) and SEM (3D surface images).
Magnification Formula:

- Units: 1 mm = 1,000 µm; 1 µm = 1,000 nm.
5. Key Processes & Terms
- Photosynthesis: Occurs in chloroplasts (chlorophyll absorbs light).
- Respiration: Occurs in mitochondria (releases energy).
- Turgidity: Vacuole presses cytoplasm against cell wall (keeps plant rigid).
- Transpiration: Water loss from leaves (drives water uptake via xylem).
Quick Revision Tips:
- Use flashcards for organelle functions.
- Practice labeling cell diagrams.
- Memorize magnification formula and unit conversions.
- Compare adaptations of specialized cells using tables.
50 GCSE Biology Questions on Cell Structure
Answers provided at the end.
Section 1: Prokaryotic vs. Eukaryotic Cells
- State three structural differences between prokaryotic and eukaryotic cells.
- Name the genetic material found in prokaryotic cells.
- What are plasmids, and in which type of cell are they found?
- Explain why prokaryotic cells are generally smaller than eukaryotic cells.
- Why do scientists believe prokaryotes evolved before eukaryotes?
Section 2: Animal and Plant Cells
- List three structures present in plant cells but absent in animal cells.
- What is the function of the vacuole in plant cells?
- Why are chloroplasts absent in root hair cells?
- Describe the role of the cellulose cell wall in plant cells.
- How does the shape of animal cells differ from plant cells?
Section 3: Specialised Cells
- Explain how a sperm cell is adapted for its function.
- Why do muscle cells contain many mitochondria?
- Describe two adaptations of red blood cells.
- How does the structure of a nerve cell support its role in transmitting impulses?
- What is the function of root hair cells, and how are they adapted?
- Why are xylem cells hollow, and how does this aid their function?
- What is the role of phloem cells, and how do sieve plates assist this process?
- Why do root hair cells lack chloroplasts?
- Explain how the biconcave shape of red blood cells increases efficiency.
- Compare the structure of xylem and phloem cells.
Section 4: Microscopy
- Calculate the total magnification if the eyepiece lens is ×10 and the objective lens is ×40.
- State two advantages of electron microscopes over light microscopes.
- Why are electron microscope images black and white?
- What is resolution, and why is it important in microscopy?
- Describe how to prepare a slide of onion epidermal cells.
- Explain why iodine solution is used when observing plant cells.
- What is the maximum resolution of a light microscope?
- How does a transmission electron microscope (TEM) differ from a scanning electron microscope (SEM)?
- Why are specimens for electron microscopes often dead?
- Name two parts of a light microscope and their functions.
Section 5: Cell Processes and Organelles
- Where in the cell does respiration occur, and what is its purpose?
- What is the function of ribosomes?
- Explain the role of chlorophyll in photosynthesis.
- Define turgidity and its importance in plants.
- How does the nucleus control cell activities?
- What is cell differentiation, and why is it important?
- Why can plant cells differentiate throughout their life, unlike animal cells?
- Describe the function of the cell membrane.
- What is transpiration, and how does it relate to xylem function?
- How do mitochondria structure (e.g., folded membranes) relate to their function?
Section 6: Calculations and Units
- Convert 5 mm to micrometres (µm).
- An image of a cell measures 45 mm. If the actual cell is 15 µm, calculate the magnification.
- A pinhead is 2 mm long. If magnified ×500, what is the image size in cm?
- If a mitochondrion is 0.5 µm in real life, how long would it appear under a ×10,000 magnification?
- Define nanometre (nm) and its relation to micrometres (µm).
Section 7: Application and Analysis
- Orchids have green roots. Suggest why this adaptation might be beneficial.
- Explain why muscle cells in the heart (cardiac muscle) have many mitochondria.
- A student observes a cell with a cell wall and chloroplasts. Is it an animal, plant, or bacterial cell? Justify.
- Why might a leaf cell have more chloroplasts than a root cell?
- A scientist claims prokaryotic cells are less complex than eukaryotic cells. Evaluate this statement.
Detailed Answers
Section 1: Prokaryotic vs. Eukaryotic Cells
- Differences: Prokaryotes lack a nucleus (DNA in a loop), are smaller (1–10 µm), and lack membrane-bound organelles.
- Genetic material: Single circular DNA loop and plasmids.
- Plasmids: Small rings of DNA; found in prokaryotes (e.g., bacteria).
- Size: Prokaryotes lack complex organelles, reducing internal space needs.
- Evolution: Fossil evidence shows prokaryotes existed ~3.5 billion years ago, earlier than eukaryotes.
Section 2: Animal and Plant Cells
- Plant-only structures: Cell wall, chloroplasts, permanent vacuole.
- Vacuole function: Stores cell sap, maintains turgidity.
- Chloroplasts in roots: Roots are underground; no light for photosynthesis.
- Cell wall role: Provides structural support and prevents bursting.
- Shape: Animal cells are irregular; plant cells are regular (due to rigid wall).
Section 3: Specialised Cells
- Sperm adaptations: Tail (movement), mitochondria (energy), streamlined shape.
- Muscle mitochondria: High energy demand for contraction.
- Red blood cells: Biconcave shape (increases surface area), no nucleus (more hemoglobin).
- Nerve cell: Long axon (transmits impulses), myelin sheath (insulates axon).
- Root hair cells: Increase surface area for water/mineral absorption; thin extension.
- Xylem: Hollow tubes (dead cells) transport water; lignin strengthens walls.
- Phloem: Transports sucrose via sieve plates; companion cells support function.
- No chloroplasts in roots: No light for photosynthesis.
- Biconcave shape: Maximizes oxygen absorption and flexibility.
- Xylem vs. phloem: Xylem is dead/hollow; phloem is alive with sieve plates.
Section 4: Microscopy
- Total magnification: ×10 × ×40 = ×400.
- Electron advantages: Higher resolution (~0.1 nm), greater magnification (×2,000,000).
- B&W images: Electrons lack color; color is added artificially.
- Resolution: Minimum distance between distinguishable points; critical for detail clarity.
- Onion slide: Peel epidermis, stain with iodine, add coverslip to avoid bubbles.
- Iodine use: Stains starch in plant cells for visibility.
- Light resolution: ~200 nm.
- TEM vs. SEM: TEM shows internal structures; SEM gives 3D surface images.
- Dead specimens: Electron beams require vacuums, killing live cells.
- Microscope parts: Eyepiece (viewing), objective (magnification), stage (holds slide).
Section 5: Cell Processes and Organelles
- Respiration: In mitochondria; releases energy (ATP) from glucose.
- Ribosomes: Site of protein synthesis.
- Chlorophyll: Absorbs light for photosynthesis.
- Turgidity: Vacuole presses cytoplasm against wall, keeping plant rigid.
- Nucleus: Contains DNA, which codes for proteins controlling cell activities.
- Differentiation: Cells specialize for specific functions (e.g., nerve cells transmit signals).
- Plant differentiation: Retain stem cells in meristems for growth/repair.
- Cell membrane: Controls substance entry/exit; selectively permeable.
- Transpiration: Water evaporates from leaves, pulling water up xylem.
- Mitochondria folds: Increase surface area for respiration enzymes.
Section 6: Calculations and Units
- 5 mm to µm: 5 × 1000 = 5000 µm.
- Magnification: 45 mm = 45,000 µm; 45,000 ÷ 15 = ×3000.
- Pinhead image: 2 mm × 500 = 1000 mm = 100 cm.
- Mitochondrion image: 0.5 µm × 10,000 = 5000 µm (5 mm).
- Nanometre: 1 nm = 0.001 µm; 1 µm = 1000 nm.
Section 7: Application and Analysis
- Green roots: Chlorophyll allows photosynthesis in light-exposed roots.
- Cardiac mitochondria: Continuous heartbeat requires constant energy.
- Cell type: Plant (bacteria lack chloroplasts; animals lack cell walls).
- Leaf chloroplasts: Leaves are photosynthetic; roots are not.
- Prokaryote complexity: Simpler due to no nucleus/organelles, but still highly efficient.
Tip: Use diagrams, flashcards, and practice calculations to reinforce these concepts!